Composite
Part:BBa_K2259035:Design
Designed by: Laurynas Karpus Group: iGEM17_Vilnius-Lithuania (2017-10-02)
Toehold activated beta-neomycin (SynORI framework)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 584
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 433
Illegal SapI.rc site found at 643
Design Notes
Mutations for group selection had to be carefully tailored in order not to change secondary structure of RNA I.
Source
This part is a modified version of wildtype ColE1 RNA I taken from addgene.